Products and Technology
Update your expectations about how accurate, scalable and affordable DNA sequencing can be. The GenapSys Sequencer is based on our novel and robust sequencing technology. Our proprietary NGS technology with proven Sequencing-by-Synthesis chemistry employs electrical-based detection of single nucleotide incorporations. With CMOS-based detectors (Complementary Metal Oxide Semiconductor), simple fluidics, and no moving parts, the GenapSys Sequencer is compact, robust, easy to use, and inexpensive.
Sequencing is carried out on microfluidic chips that have a scalable number of detectors, allowing for applications ranging from targeted sequencing of specific amplicons to genome-scale data collection. The system utilizes the first ever purely electrical sequencing chip, the key component in our fully integrated proprietary platform.
The platform is capable of generating high-quality sequence data and delivers the high resolution and analytical sensitivity needed for detection of rare variants and transcripts. It routinely generates sequence data that exceeds >80% of bases >Q30 with average read lengths of >150 bp. An example of GenapSys’ high-quality data from a genomic library, generated at an external lab, is shown below.
1.6Gb run output, 11.2M reads
Shifting from optics to electrical based detection creates a substantial reduction in capital cost. The simplicity of the detection modality that does not require any optics, robots, scanners, or expensive analysis servers, allows for a lowcost and affordable instrument. The instrument list price puts sequencing within the reach of virtually any lab. The GenapSys Sequencer provides an affordable solution for Next-Generation Sequencing in labs of all types.
The low per-run cost means reasonable cost per sample without excessive sample batching. Researchers can now dictate the pace of their work by performing runs at their own convenience, without having to wait for additional samples to be batched before starting a run. Additionally, researchers can maintain control of the sequencing process from beginning to end, providing higher confidence in sample integrity and data analysis results.
Our sequencing platform offers the built-in capability to easily scale up from 1 million to 16 million to 144 million sensors, simply by utilizing a different sequencing chip, all of which are capable of running on the same affordable instrument.
Our 3 chips enable a wide range of throughputs suitable for various applications, providing flexibility for the customers without the need of excessive sample batching. For example, our 16M sensor chip is capable of generating 1.2-2.0 Gb of high quality sequence data with average read lengths of >150 bp.
G2-1M Sensor Chip
G3-16M Sensor Chip
G4-50M Sensor Chip
(Available in 2021)
At the heart of our system are CMOS chips (Complementary Metal Oxide Semiconductor), the first chips that deliver purely electrical-based sequencing. Shifting from optical to electrical sequencing provides a cost-effective, scalable solution without the need for excessive sample batching.
With the option of 1 million, 16 million or 144 million-sensor chips, you can scale up your throughput whenever you want, without changing the underlying instrument. Sequence anything from cancer panels to whole human genomes simply by loading the right chip.
The GenapSys Sequencing Prep System replaces the manual clonal amplification step in the GenapSys Sequencer workflow with a load-and-go process that automates template preparation and enrichment in one device. This small-footprint instrument uses cartridge-based reagents, and employs magnetic bead technology to isolate template-positive particles that can be subsequently loaded onto GenapSys sequencing chips.
Product | Catalog No. | Description |
GenapSys™ Sequencer | 1001500 | Sequencer and accessories. Comes with a standard 1-year warranty that includes depot repair, all parts and labor. |
GenapSys™ Sequencing Prep System | 1002470 | System for automated clonal amplification and enrichment for the GenapSys Sequencer. |
G3 Sequencing Kit V3, Dual Index | 1001975 | Kitted reagents and consumables for a single dual-index sequencing run. Includes a single ready-to-use sequencing cartridge and one G3 sequencing chip. |
G3 Sequencing Kit V3, No Index | 1001977 | Kitted reagents and consumables for a single non-index sequencing run. Includes a single ready-to-use sequencing cartridge and one G3 sequencing chip. |
G3 Amplification Kit V3 | 1001438 | Kitted reagents and consumables for a single run of automated amplification on the Sequencing Prep System. |
GenapSys™ has developed a novel electrical-based platform capable of accurately detecting single base incorporations. On the surface of the CMOS sequencing chip (Complementary Metal Oxide Semiconductor), there are millions of sensors, each designed to capture one clonally amplified bead. Every sensor consists of electrodes in close proximity to each other, both of which contact the bead captured by that sensor.
Since nucleotide incorporation into a growing DNA strand increases the amount of charged molecules around the bead, it changes the impedance measured by the sensor. The magnitude of the impedance change is correlated to the total change in the charge of DNA molecules and their surrounding layers around the bead, which is correlated to the number of incorporated nucleotides.
The plot contains a representative example of acquired data showing the distribution of measured differential signal across multiple nucleotide flows for a single template sequence. Flows that are expected to have nucleotide incorporations are highlighted in red. Non-incorporating flows are shown in blue and do not deviate significantly from the baseline. The inferred sequence, including the number of nucleotides in each incorporation are shown below.
While almost all other NGS technologies are based on transient measurements, our detection modality is based on steady state measurements. Due to its steady state nature, the signal stays constant and we can take multiple measurements to increase our precision and signal-to-noise (SNR) which significantly improves our base calling accuracy. Also, the design of the chip allows for very low cross-talk between the sensors which enables high density packing of the sensors.