Update your expectations about how accurate, scalable and affordable DNA sequencing can be. The GenapSys Sequencer is based on our novel and robust sequencing technology. Our proprietary NGS technology with proven Sequencing-by-Synthesis chemistry employs electrical-based detection of single nucleotide incorporations. With CMOS-based detectors (Complementary Metal Oxide Semiconductor), simple fluidics, and no moving parts, the GenapSys Sequencer is compact, robust, easy to use, and inexpensive.
Sequencing is carried out on microfluidic chips that have a scalable number of detectors, allowing for applications ranging from targeted sequencing of specific amplicons to genome-scale data collection. The system utilizes the first ever purely electrical sequencing chip, the key component in our fully integrated proprietary platform.
Exceptional Accuracy: >80% of bases >Q30 validated by numerous applications in experienced third party labs.
The platform is capable of generating high-quality sequence data and delivers the high resolution and analytical sensitivity needed for detection of rare variants and transcripts. It routinely generates sequence data that exceeds >80% of bases >Q30 with average read lengths of >150 bp. An example of GenapSys’ high-quality data from a genomic library, generated at an external lab, is shown below.
Externally generated data with >80% of bases >Q30
1.6Gb run output, 11.2M reads
Unrivaled Scalability: A range of outputs tuned to your sample throughput needs.
Our sequencing platform offers the built-in capability to easily scale up from 1 million to 16 million to 144 million sensors, simply by utilizing a different sequencing chip, all of which are capable of running on the same affordable instrument.
Our 3 chips enable a wide range of throughputs suitable for various applications, providing flexibility for the customers without the need of excessive sample batching. For example, our 16M sensor chip is capable of generating 1.2-2.0 Gb of high quality sequence data with average read lengths of >150 bp.
Amazing Affordability: Low capital costs, low operating costs.
Shifting from optics to electrical based detection creates a substantial reduction in capital cost. The simplicity of the detection modality that does not require any optics, robots, scanners, or expensive analysis servers, allows for a lowcost and affordable instrument. The instrument list price puts sequencing within the reach of virtually any lab. The GenapSys Sequencer provides an affordable solution for Next-Generation Sequencing in labs of all types.
The low per-run cost means reasonable cost per sample without excessive sample batching. Researchers can now dictate the pace of their work by performing runs at their own convenience, without having to wait for additional samples to be batched before starting a run. Additionally, researchers can maintain control of the sequencing process from beginning to end, providing higher confidence in sample integrity and data analysis results.
Semiconductor chips enable scalable and affordable sequencing.
1M sensor chip
16M sensor chip
144M sensor chip
(Available in 2020)
At the heart of our system are CMOS chips (Complementary Metal Oxide Semiconductor), the first chips that deliver purely electrical-based sequencing. Shifting from optical to electrical sequencing provides a cost-effective, scalable solution without the need for excessive sample batching.
With the option of 1 million, 16 million or 144 million-sensor chips, you can scale up your throughput whenever you want, without changing the underlying instrument. Sequence anything from cancer panels to whole human genomes simply by loading the right chip.
1. Library Preparation
The sequencer is compatible with a broad range of library preparation methodologies. Performance has been demonstrated across multiple kits.
2. Clonal Amplification
Addition of pooled libraries and run consumables is followed by a simple run setup process. Once in operation, the GenapSys Sequencing Prep System automates the clonal amplification procedure in an unattended 4-hour run. The final amplified product is then ready for sequencing.
Amplified material is loaded into a sequencing chip. The user-friendly system interface walks the user through consumables loading and the run setup procedure. The system uses our proprietary high accuracy SBS chemistry to deliver high-quality data.
4. Data Analysis
The sequencing run output is a standard FASTQ file. This allows for existing secondary analysis tools to be utilized for analysis of GenapSys data. For highest quality results, we recommend customers to use GenHub, our proprietary cloud hosted solution, to run secondary analyses with empirically derived, optimized parameters for GenapSys data. This obviates the need for expensive additional localized servers. Additionally GenHub provides customers real time monitoring of the status of the sequencing run as well as accessing FASTQ and VCF files of all runs.
The GenapSys Sequencing Prep System replaces the manual clonal amplification step in the GenapSys Sequencer workflow with a load-and-go process that automates template preparation and enrichment in one device. This small-footprint instrument uses cartridge-based reagents, and employs magnetic bead technology to isolate template-positive particles that can be subsequently loaded onto GenapSys sequencing chips.
|GenapSys™ Sequencer||1001500||Sequencer and accessories. Comes with a standard 1-year warranty that includes depot repair, all parts and labor.|
|GenapSys™ Sequencing Prep System||1000712||System for automated clonal amplification and enrichment for the GenapSys Sequencer.|
|G3 Sequencing Kit||1001587||Kitted reagents and consumables for a single sequencing run. Includes a single ready-to-use sequencing cartridge and one G3 sequencing chip.|
|G3 Amplification Kit||1001588||Kitted reagents and consumables for a single run of automated amplification on the Sequencing Prep System.|
GenapSys™ has developed a novel electrical-based platform capable of accurately detecting single base incorporations. On the surface of the CMOS sequencing chip (Complementary Metal Oxide Semiconductor), there are millions of sensors, each designed to capture one clonally amplified bead. Every sensor consists of electrodes in close proximity to each other, both of which contact the bead captured by that sensor.
Since nucleotide incorporation into a growing DNA strand increases the amount of charged molecules around the bead, it changes the impedance measured by the sensor. The magnitude of the impedance change is correlated to the total change in the charge of DNA molecules and their surrounding layers around the bead, which is correlated to the number of incorporated nucleotides.
The plot contains a representative example of acquired data showing the distribution of measured differential signal across multiple nucleotide flows for a single template sequence. Flows that are expected to have nucleotide incorporations are highlighted in red. Non-incorporating flows are shown in blue and do not deviate significantly from the baseline. The inferred sequence, including the number of nucleotides in each incorporation are shown below.
Our steady state electrical signal increases signal-to-noise ratio for greater base-calling accuracy.
While almost all other NGS technologies are based on transient measurements, our detection modality is based on steady state measurements. Due to its steady state nature, the signal stays constant and we can take multiple measurements to increase our precision and signal-to-noise (SNR) which significantly improves our base calling accuracy. Also, the design of the chip allows for very low cross-talk between the sensors which enables high density packing of the sensors.